Which techniques are used to detect RNA sequences?

Northern blotting is the technique specifically designed for detecting RNA sequences. This method involves the separation of RNA samples through gel electrophoresis, followed by transfer to a membrane and subsequent probing with labeled complementary DNA or RNA probes. The presence of specific RNA sequences is then visualized, allowing for analysis of gene expression, RNA size, and RNA processing.

In contrast, Western blotting is utilized for the detection of proteins, and Southern blotting is used for DNA. ELISA (Enzyme-Linked Immunosorbent Assay) primarily detects proteins or antibodies rather than nucleic acids. Each of these techniques serves distinct purposes in molecular biology, highlighting the specificity of Northern blotting for RNA analysis.